Product: Bromfenac (sodium hydrate)
Influenza B Virus Nucleoprotein Antibody (3E9/B2) – BSA Free Summary
| Immunogen |
Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37C.
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| Epitope |
Epitope differs from Clone 03 as determined by inhibition ELISA
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| Specificity |
Clone 3E9/B2 reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. Clone 3E9/B2 does not cross-react with influenza A virus.
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| Isotype |
IgG2a Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Clone 3E9/B2 is suitable as capture antibody in combination with biotinylated Clone 03 as detecting antibody in sandwich ELISA for influenza B virus.Clone 3E9/B2 reacts with influenza B-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.In Western blotting a dilution guideline of 1/20 has proved successful.
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Reactivity Notes
Influenza B virus
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
10mM Phosphate (pH 7.4) and 0.5M NaCl
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| Preservative |
15mM Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Influenza B Virus Nucleoprotein Antibody (3E9/B2) – BSA Free
- Influenza B Virus NP
- NP
- Nucleoprotein
Background
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle