Imm-Link BSA (Sulfhydryl) Immunogen labeling Kit

Product: DPN

Imm-Link BSA (Sulfhydryl) Immunogen labeling Kit Summary

Description
BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.

The imm-Link sulfhydryl conjugation kit allows sulfhydryl containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.

Following successful conjugation the conjugate is dialysed in the supplied dialysis cartridge to remove unwanted by products. The hapten conjugate can then be easily recovered and used.

Immunogen Kit Sulfhydryl
Target Functional Groups Cysteines or free sulfhydryl groups (SH)

Features:

  • Easy to use
  • All components supplied
  • Wide range of chemistries
  • Hands on time to set up conjugation reaction is 20-30 seconds
  • Supplied dialysis cartridge ensures conjugates are recovered in high yield
  • Full technical support available
Kit Type
Immunogen labeling Kit

Applications/Dilutions

Application Notes
Target Functional Groups: Cysteines or free sulfhydryl groups (SH).
Reviewed Applications
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using
462-0001 in the following application:

  • ELISA

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 1 or 3 dialysis cartridge(s)
  2. 1 or 3 glass vial(s) of Imm-Link mix
  3. 1 or 3 250 ml bottle(s) of 10x dialysis buffer
  4. 1 or 3 vial(s) of Imm-Link Sulfhydryl Modifier reagent

Notes

Imm-Link® is a registered trademark of Innova Biosciences.

PMID: 6548377