IgG Antibody (SPM566) – Azide and BSA Free Summary
Immunogen |
Purified human Ig Gamma Chain
|
Localization |
Cytoplasm, Cell Surface and Secreted
|
Marker |
B Cell Marker
|
Specificity |
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
|
Isotype |
IgG2a Kappa
|
Clonality |
Monoclonal
|
Host |
Mouse
|
Gene |
IGHG1
|
Purity |
Protein A purified
|
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|
Applications/Dilutions
Dilutions |
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Application Notes |
Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
Buffer |
10mM PBS – BSA free
|
Preservative |
No Preservative
|
Concentration |
1.0 mg/ml
|
Purity |
Protein A purified
|
Alternate Names for IgG Antibody (SPM566) – Azide and BSA Free
- IgG
- immunoglobulin heavy constant gamma 1 (G1m marker)