INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues Summary
| Description |
Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
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| Background |
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Skeletal Muscle, Heart, Kidney Tissues NBP2-30116 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. Weve streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS…-PAGE and electro-blotting steps. With INSTA-Blot™ immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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| Preparation Method |
INSTA-Blot™ Multi-species Skeletal Muscle, Heart, Kidney Tissue Lysate NBP2-30116 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4- 20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot ™ is provided ready-to-use in your western blot protocol.
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Applications/Dilutions
| Application Notes |
Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% b-mercaptoethanol).
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Packaging, Storage & Formulations
| Storage |
Store at room temperature.
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Notes
The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.
Alternate Names for INSTA-Blot Multi-species Skeletal Muscle, Heart and Kidney Tissues
- INSTA Blot Multi-species Skeletal Muscle, Heart, Kidney Tissues
- INSTABlot Multi-species Skeletal Muscle, Heart, Kidney Tissues
- INSTA-Blot Multi-species Skeletal Muscle, Heart, Kidney