IL-3 R beta Antibody (130705) [Alexa Fluor® 647] Summary
Specificity |
Detects mouse IL‑3 R beta in direct ELISAs. Cross-reactivity with AIC2B was not tested.
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Isotype |
IgG2a
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Clonality |
Monoclonal
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Host |
Rat
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Gene |
Csf2rb2
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Applications/Dilutions
Dilutions |
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Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.
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Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 – 8 °C. Do not use past expiration date.
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Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
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Preservative |
0.09% Sodium Azide
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Concentration |
Please see the vial label for concentration. If unlisted please contact technical services.
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Notes
Alternate Names for IL-3 R beta Antibody (130705) [Alexa Fluor® 647]
- AIC2A
- BetaIl3
- Bil3
- Csfgmrb
- IL-3 R beta
- IL3R beta
- IL3R
- IL3RB
- IL-3Rb
- Il3rb2
Background
Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha and the beta subunits are members of the cytokine receptor superfamily (3).