IL-37/IL-1F7 Antibody (261506) [Unconjugated] Summary
| Immunogen |
E. coli-derived recombinant human IL-37/IL-1F7
Lys27-Asp192 Accession # NP_775297 |
| Specificity |
Detects human IL-37/IL-1F7 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-36Ra/IL-1F5, rhIL-36 alpha/IL-1F6, rhIL-36 beta/IL-1F8, or rhIL-18/IL-1F4 is observed.
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| Source |
N/A
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| Isotype |
IgG2b
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
IL37
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| Endotoxin Note |
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
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| Preservative |
No Preservative
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| Concentration |
LYOPH
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| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS.
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Notes
Alternate Names for IL-37/IL-1F7 Antibody (261506) [Unconjugated]
- FIL1 zeta
- FIL1
- FIL1(ZETA)
- FIL1ZFIL1 zeta
- IL-1F7
- IL-1F7IL-1 zeta
- IL1H4IL1F7 (canonical product IL-1F7b)
- IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1)
- IL-1RP1IL-1X protein
- IL1RP1interleukin 1 family member 7
- IL-1X
- IL37
- IL-37
- interleukin 1 family, member 7 (zeta)
- interleukin 1, zeta
- interleukin-1 family member 7
- Interleukin-1 homolog 4
- interleukin-1 superfamily z
- Interleukin-1 zeta
- Interleukin-1-related protein
Background
Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).