IFN-gamma R1/CD119 Antibody (BB1E-2) Summary
| Immunogen |
Fusion protein of the extracellular part of the human interferon-gamma receptor (AA: Ala17-Gly245) to 11 AA of the c-myc proto-oncogene (for affinity purification).
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| Specificity |
In Western Blotting only one protein band is stained corresponding to a MW of 43,000, which is equal to the MW for the glycosylated extracellular part of the human extra-cellular interferon-gamma receptor (fused to c-myc), and a MW of 27,000 corresponding to the non-glycosylated part (fused to c-myc). The antibody does not neutralise biological activity of interferon gamma.
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
IFNGR1
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| Purity |
Protein A or G purified
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Applications/Dilutions
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| Application Notes |
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
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| Positive Control |
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| Publications |
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Reactivity Notes
Reacts with Human. Cross reacts with Bovine.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.2)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for IFN-gamma R1/CD119 Antibody (BB1E-2)
- AVP, type 2
- CD119 antigen
- CD119
- FLJ45734
- IFN-gamma R1
- IFN-gamma receptor 1
- IFNgammaR1
- IFN-gamma-R1
- IFNGR
- IFNGR1
- IFN-gR1
- immune interferon receptor 1
- interferon gamma receptor 1
- interferon-gamma receptor alpha chain
- type 2
Background
The IFNG receptor complex is composed of two type I membrane proteins, IFNGR1 (IFNGR alpha) and IFNGR2 (IFNGR beta). Both receptor proteins are members of the type II cytokine receptor family and share 52% overall sequence identity. The IFNG receptor is constitutively expressed in most cell types. Soluble IFNG has been detected in biological fluids. IFNGR1 is required for ligand-binding and signaling. It binds to IFNG with high affinity and is a potent IFNG antagonist. In signal transduction, IFNG induces tyrosine phosphorylation of IFNGR1 leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFNG-induced gene transcription. Human IFNGR1, a 227 amino acid residue protein, has a calculated molecular mass of approximately 25 kDa. As a result of glycosylation, the recombinant IFNGR1 migrates as a 40 to 50 kDa protein in SDS-PAGE. Human and mouse IFNGRI share approximately 52% amino acid sequence identity and each interacts with IFNG in a strictly species-specific manner.