ICAM-3/CD50 Antibody (ICAM3/1019)

Product: MMAF (Hydrochloride)

ICAM-3/CD50 Antibody (ICAM3/1019) Summary

Immunogen
Recombinant human ICAM3 protein
Localization
Cell surface
Specificity
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ICAM3
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 – 1.0 ug/ml
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 – 1.0 ug/ml
  • Immunofluorescence 0.5 – 1.0 ug/ml
Application Notes
Hu-chromosome location: 19p13.2
Molecular weight of antigen: 110-160kDa
Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for ICAM-3/CD50 Antibody (ICAM3/1019)

  • CD50 antigen
  • CD50
  • CDW50CDw50
  • ICAM3
  • ICAM-3
  • ICAM-Rintercellular adhesion molecule-3
  • intercellular adhesion molecule 3

PMID: 23527575