Huntingtin Antibody (HDC8A4) Summary
| Immunogen |
Recombinant fragment, corresponding to amino acids 2703-2911 of Huntingtin.
|
| Localization |
Cytoplasmic
|
| Specificity |
This reacts with an epitope corresponding to the HDC region (2703 – 2911 amino acids) of the huntingtin protein
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
HTT
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
||
| Application Notes |
Immunohistology – frozen (paraffin not tested)Western blotting – detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
|
||
| Positive Control |
|
Reactivity Notes
Cross-reacts with Human, Rabbit and Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffered saline
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for Huntingtin Antibody (HDC8A4)
- HD protein
- huntingtin (Huntington disease)
- huntingtin
- IT15HDHuntington disease protein
Background
The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain.