Human lambda light chain Antibody (K22-Y) Summary
| Immunogen |
Peptide derived from N-terminal sequence of human lambda light chain.
|
| Specificity |
This antibody is specific for the N terminus of the lambda light chain.
|
| Isotype |
IgG
|
| Clonality |
Monoclonal
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| Host |
Rabbit
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| Gene |
IGLC1
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
For use in Immunohistochemistry frozen and paraffin embedded sections. For 0.1ml, 0.5ml and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted.
|
Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Notes
The 7mL and 15mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Alternate Names for Human lambda light chain Antibody (K22-Y)
- Bence Jones protein
- BJP
- Constant region of lambda light chains
- HCAK 1
- HCAK1
- Ig kappa chain C region
- Ig lambda chain C regions
- IGKC
- IGLC 1
- IGLC 2
- IGLC 3
- IGLC
- IGLC1
- IGLC2
- IGLC3
- Immunoglobulin kappa constant region
- Immunoglobulin kappa constant
- Immunoglobulin kappa light chain
- Immunoglobulin lambda constant 1
- Kappa 1 immunoglobulin light chain
- Km
- Mcg marker
- MGC111575
- MGC62011
- MGC72072
- MGC88770
- MGC88771
- MGC88809
- Paraprotein
Background
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain are present in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).