Human Osteopontin/OPN Biologically Active Protein Summary
| Description |
A biologically active protein with a C-terminal 6-His tag corresponding to amino acids Ile17 – Asn300 to SPP1.
Source: Mouse myeloma cell line, NS0 |
| Preparation Method |
Lyophilized from a 0.2 um filtered solution in PBS.
|
| Details of Functionality |
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Recombinant Human (rh) Osteopontin/OPN immobilized at 1 ug/mL, 100 uL/well, will mediate the adhesion of added 293 cells >30% (1 x 105 cells/well). Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rhOsteopontin/his can increase HEK293 cell adhesion by about 5-fold.
|
| Protein/Peptide Type |
Biologically Active Protein
|
| Gene |
SPP1
|
| Endotoxin Note |
<0.10 EU per 1 ug of the protein by the LAL method.
|
Applications/Dilutions
| Theoretical MW |
32.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Concentration |
LYOPH
|
| Reconstitution Instructions |
Reconstitute with 0.1 mg/ml in sterilized PBS to desired concentration.
|
Notes
This product is provided in a Carrier Free format and does NOT contain BSA. This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Human Osteopontin/OPN Biologically Active Protein
- BNSP
- Bone sialoprotein 1
- Eta-1
- MGC110940
- Nephropontin
- OPN
- Osteopontin
- secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
- secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
- Spp1
- SPP-1
- SPP1/CALPHA1 fusion
- Urinary stone protein
- uropontin