Human Duodenum Whole Tissue Lysate (Adult Whole Normal) Summary
| Specificity |
Total Protein – Human Adult Normal Tissue: Small Intestine: Duodenum
|
Applications/Dilutions
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
|
Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors
|
| Concentration |
5.0 mg/ml
|
Lysate Details for Duodenum
| Type |
Tissue
|
| Life Stage |
Adult
|
| Tissue |
Digestive/Waste
|
| Protein State |
Native
|
| Tissue Condition |
Normal
|
| Subcellular Fraction |
Whole
|
Notes
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.