Human CD4 T Cell Isolation Kit Summary
| Description |
The MagCellect Human CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD4+ T cells. Typical recovery ranges from 45-65% and the purity of recovered CD4+ T cells ranges from 90-97%, with no detectable CD8+ T cells. Key Benefits:
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| Kit Type |
Isolation Kit
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| Gene |
CD4
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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Kit Components
| Components |
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Notes
Why Isolate Regulatory T Cells: CD4+ T cells develop in the thymus and differentiate into various subsets of more specialized T lymphocytes. The development of these subsets is induced by distinct signals and is controlled by distinct transcription factors. CD4+ T cell subsets produce characteristic combinations of cytokines which enables them to exert diverse functions. CD4+ T cells are critically involved in recruiting and activating other immune cells, dampening ongoing immune responses, and maintaining immunologic memory. The relative expansion of CD4+ subsets changes dramatically in response to local environmental stresses or disease status. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, and Th17 cells. Precaution: Some components of this kit contain Sodium Azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Other Supplies Required: MagCellect Magnet, 12 x 75 mm (5 ml) or 17 x 100 mm (15 ml) polystyrene round bottom tubes, Sterile Pasteur pipettes or transfer pipettes or equivalent
Background
CD4 is a transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th9, Th17,Th22, Tfh, and Treg cells which regulate humoral and cellular immunity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1. MagCellect products are designed for the isolation of cells in a “liquid phase”. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.