Product: PF-CBP1 (hydrochloride)
Histone H3 [ac Lys9, p Ser10] Antibody Summary
Immunogen |
Synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.
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Modification |
ac Lys9, p Ser10
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Specificity |
Synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3. Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide.
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Clonality |
Polyclonal
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Host |
Rabbit
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Gene |
HIST3H3
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Purity |
IgG purified
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Innovators Reward |
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Applications/Dilutions
Dilutions |
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Application Notes |
ChIP:Approximately 4-5 ug of each antibody was used with 30-40 ug of chromatin per ChIP.IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use.
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Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles.
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Buffer |
10mM PBS
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Preservative |
15mM Sodium Azide
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Purity |
IgG purified
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Alternate Names for Histone H3 [ac Lys9, p Ser10] Antibody
- H3 histone, family 3A
- H3.3AH3F3H3F3B
- H3.3B
- H3F3A
- H3K9ac
- Histone H3
- histone H3.3
- MGC87782
- MGC87783