Haptoglobin Antibody

Product: SGI-1776

Haptoglobin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Haptoglobin. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 – 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (81%), Rat (80%), Feline (83%), Porcine (81%), Rabbit (86%), Rhesus Monkey, Chimpanzee (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.59 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Haptoglobin Antibody

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141

Background

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohns disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinsons disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

PMID: 24494685

Haptoglobin Antibody

Product: K03863

Haptoglobin Antibody Summary

Immunogen
Peptide with sequence C-STVPEKKTPKSP corresponding to internal region according to NP_005134.1, NP_001119574.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_005134.1; NP_001119574.1).
Clonality
Polyclonal
Host
Goat
Gene
HP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 – 0.3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 37+40 kDa band bands observed in human liver lysates and 40 kDa band in porcine liver lysates (calculated MW of 45.2 kDa band according to NP_005134.1 and 38.5 kDa band according to NP_001119574.1).
Publications
Read Publication using NBP1-97756.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Haptoglobin Antibody

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141

PMID: 9489602