H-2Dd Antibody (34-5-8S) [Biotin] Summary
| Immunogen |
BDF1 mouse splenocytes
|
| Specificity |
A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Protein A or G purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Readout System |
|
Reactivity Notes
Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS
|
| Preservative |
Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Protein A or G purified
|
Notes
Protect conjugated forms from light.
Alternate Names for H-2Dd Antibody (34-5-8S) [Biotin]
- H2Dd
- MHC class I
Background
MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block therecognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)