HSP70/HSPA1A Antibody

Product: Bretylium (tosylate)

HSP70/HSPA1A Antibody Summary

Immunogen
Hsp70Full length
Localization
Cytoplasm
Specificity
Detects approx 70kDa.
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
HSPA1A
Purity
PEG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
Application Notes
1 ug/ml of HSP70 Antibody was sufficient for detection of HSP70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-chicken IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, Its predicted to detect Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
PEG purified

Alternate Names for HSP70/HSPA1A Antibody

  • dnaK-type molecular chaperone HSP70-1
  • FLJ54303
  • FLJ54370
  • FLJ54392
  • FLJ54408
  • FLJ75127
  • Heat shock 70 kDa protein 1/2
  • heat shock 70 kDa protein 1A/1B
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
  • heat shock-induced protein
  • HSP70
  • HSP70.1/HSP70.2
  • HSP70-1
  • HSP70-1/HSP70-2
  • HSP70-1A
  • HSP70I
  • HSP72
  • HSPA1
  • HSPA1A
  • HSPA1B

Background

Certificate of Analysis, 1.0 ug/mL was sufficient for detection of Hsp70 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-chicken IgG: HRP as the secondary antibody.

PMID: 23261596