HMGN3/TRIP7 Antibody

Product: HMN-176

HMGN3/TRIP7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 30 and 60 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324).
Clonality
Polyclonal
Host
Rabbit
Gene
HMGN3
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HMGN3/TRIP7 Antibody

  • DKFZp686E20226
  • high mobility group nucleosomal binding domain 3
  • high mobility group nucleosome-binding domain-containing protein 3
  • high-mobility group protein HMGN3
  • PNAS-25
  • thyroid hormone receptor interacting protein 7
  • thyroid hormone receptor interactor 7
  • Thyroid receptor-interacting protein 7
  • TR-interacting protein 7
  • TRIP-7
  • TRIP7FLJ42187

Background

HMGN3 was originally identified in a screen for interaction partners of the thyroid hormone receptor. HMGN3 belongs to the HMGN family of small basic proteins that interacts specifically with nucleosomes and functions to facilitate chromatin unfolding to allow access of transcription factors to DNA targets. HMGN has been found to be a regulator of the glycine transporter 1 (GLYT1) gene.

PMID: 1654255

HMGN3/TRIP7 Antibody

Product: Endoxifen (E-isomer)

HMGN3/TRIP7 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 60 and 99 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324).
Clonality
Polyclonal
Host
Rabbit
Gene
HMGN3
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 2-5ug/mg lysate

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HMGN3/TRIP7 Antibody

  • DKFZp686E20226
  • high mobility group nucleosomal binding domain 3
  • high mobility group nucleosome-binding domain-containing protein 3
  • high-mobility group protein HMGN3
  • PNAS-25
  • thyroid hormone receptor interacting protein 7
  • thyroid hormone receptor interactor 7
  • Thyroid receptor-interacting protein 7
  • TR-interacting protein 7
  • TRIP-7
  • TRIP7FLJ42187

Background

HMGN3 was originally identified in a screen for interaction partners of the thyroid hormone receptor. HMGN3 belongs to the HMGN family of small basic proteins that interacts specifically with nucleosomes and functions to facilitate chromatin unfolding to allow access of transcription factors to DNA targets. HMGN has been found to be a regulator of the glycine transporter 1 (GLYT1) gene.

PMID: 19211900

HMGN3/TRIP7 Antibody

Product: IDO-IN-6

HMGN3/TRIP7 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:KPEPKPRKTSAKKEPGAKISRGAKGKKEEKQEAGKEGTAPSENGETKAEEAQKTESVDNEGE
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGN3
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 – 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:20
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
Control Peptide
HMGN3/TRIP7 Protein (NBP1-86904PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (84%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HMGN3/TRIP7 Antibody

  • DKFZp686E20226
  • high mobility group nucleosomal binding domain 3
  • high mobility group nucleosome-binding domain-containing protein 3
  • high-mobility group protein HMGN3
  • PNAS-25
  • thyroid hormone receptor interacting protein 7
  • thyroid hormone receptor interactor 7
  • Thyroid receptor-interacting protein 7
  • TR-interacting protein 7
  • TRIP-7
  • TRIP7FLJ42187

PMID: 7578057