HLA DRB1 Antibody (SPM423) – IHC-Prediluted Summary
| Immunogen |
Activated human peripheral blood mononuclear cells
|
| Localization |
Cell Surface
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| Specificity |
This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
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| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 6p21.3 Molecular weight of antigen: 28kDa (beta chain) Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.
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Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for HLA DRB1 Antibody (SPM423) – IHC-Prediluted
- Clone P2-beta-3
- DR1
- DR-1
- DR12
- DR-12
- DR13
- DR-13
- DR14
- DR-14
- DR16
- DR-16
- DR4
- DR-4
- DR5
- DR-5
- DR7
- DR-7
- DR8
- DR-8
- DR9
- DR-9
- DRB1
- DRw10
- DRw11
- DRw8
- DW2.2/DR2.2
- FLJ75017
- FLJ76359
- HLA class II antigen beta chain
- HLA class II histocompatibility antigen, DR-1 beta chain
- HLA-DR1B
- HLA-DRB
- HLA-DRB1*
- HLA-DRB2
- HLA-DR-beta 1
- human leucocyte antigen DRB1
- leucocyte antigen DR beta 1 chain
- leucocyte antigen DRB1
- lymphocyte antigen DRB1
- major histocompatibility complex, class II, DR beta 1
- MHC class II antigen DRB1*1
- MHC class II antigen DRB1*10
- MHC class II antigen DRB1*11
- MHC class II antigen DRB1*12
- MHC class II antigen DRB1*13
- MHC class II antigen DRB1*14
- MHC class II antigen DRB1*15
- MHC class II antigen DRB1*16
- MHC class II antigen DRB1*3
- MHC class II antigen DRB1*4
- MHC class II antigen DRB1*7
- MHC class II antigen DRB1*8
- MHC class II antigen DRB1*9
- MHC class II antigen HLA-DR13
- MHC class II HLA-DR beta 1 chain
- MHC class II HLA-DR-beta cell surface glycoprotein
- MHC class II HLA-DRw10-beta
- SS1