HIST1H2AC Antibody Summary
| Immunogen |
Synthetic peptide corresponding to the N-terminus of human histone H2A.
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| Specificity |
It does not cross-react with other histones.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
HIST1H2AC
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-30 ug/ml). However, the optimal conditions should be determined individually. The antibody detects ~14 kDa histone H2A protein. It does not cross-react with other histones. HeLa cell nuclear extract can be used as a positive control.
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
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| Preservative |
0.01% Thimerosal
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for HIST1H2AC Antibody
- dJ221C16.4
- H2A/l
- H2AFLmember L
- histone 1, H2ac
- histone cluster 1, H2ac
- histone H2A type 1-C
- Histone H2A/l
- histone H2AC
- MGC99519
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.