Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC]

Product: BI-7273

Goat anti-Mouse IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Mouse IgG-heavy and light chain
Localization
Secreted
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 – 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 – 1:50
  • Immunohistochemistry 1:50 – 1:50
  • Immunohistochemistry-Paraffin 1:50 – 1:500
Application Notes
Immunohistochemistry-Paraffin and Immunocytochemistry. Flow Cytometry was reported in scientific literature.
Reviewed Applications
Read 4 Reviews rated 4

using
NB720-F in the following applications:

  • Immunohistochemistry-Paraffin
  • Immunohistochemistry-Frozen
  • Immunocytochemistry
  • Flow Cytometry
Publications
Read Publications using
NB720-F in the following applications:

  • FLOW
    1 publication
  • ICC/IF
    2 publications
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS containing 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10-11 variants.

PMID: 7608899

Goat anti-Mouse IgG (H+L) Secondary Antibody [FITC]

Product: Meropenem (trihydrate)

Goat anti-Mouse IgG (H L) Secondary Antibody [FITC] Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on mouse IgG and light chains on all mouse immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

PMID: 17904591