Goat anti-Mouse IgG Fc fragment Secondary Antibody [PE] Summary
| Immunogen |
Goat Anti-Mouse IgG (H+L)-FITC Conjugate
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| Specificity |
This antibody is specific to the Fc gamma fragment. Minimal Cross Reactivity: Human, Bovine, Rabbit Serum
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| Clonality |
Polyclonal
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| Host |
Goat
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody conjugate has been tested by FACS analysis at 0.5 ul / one million cells to assure specificity and reactivity. Since applications vary, investigators could try 1:50 to 1:200 for most applications.
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
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| Buffer |
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 containing 15 mg BSA/ml.
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| Preservative |
0.05% Sodium Azide
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| Concentration |
LYOPH
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| Purity |
Immunogen affinity purified
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| Reconstitution Instructions |
Reconstitute with 1.0 ml distilled water.
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Background
Secondary antibodies are antibodies that bind to primary antibodies or antibody fragments. They are typically labeled with probes that make them useful for detection, purification or cell sorting applications. Secondary antibodies may be polyclonal or monoclonal, and are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. Secondary antibodies are selected according to the source of the primary antibody, the class of the primary antibody (e.g., IgG or IgM), and the kind of label which is preferred. Identifying the optimal secondary antibody is normally done through trial and error method. Secondary antibodies are used in all types of immunoassays, most often in Western blot, immunohistochemistry, and immunocytochemistry, and occasionally in immunoprecipitation. Basic research, clinical analysis, and disease diagnosis also use secondary antibodies in ELISA and flow cytometry assays. They are also useful for cell sorting, fluorescence activated cell sorting, FACS. Secondary antibodies are available in a variety of formats and conjugate types. These many options provide for excellent performance in many kinds of antibody-based detection and assay techniques. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. The 20103 antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. The antibody reacts with mouse IgG subclasses, 1, 2a, 2b and 3; but not with the Fab portion of mouse immunoglobins. No antibody was detected against mouse IgM or non-immunoglobulin serum proteins. Antibody/PE ratio: 1:1 Minimal cross-reaction to human, bovine, horse and rabbit serum proteins.