Goat anti-Human IgE Secondary Antibody [DyLight 650] (Pre-absorbed)

Product: LX-4212

Goat anti-Human IgE Secondary Antibody [DyLight 650] (Pre-absorbed) Summary

Immunogen
Purified human IgE, (e chain)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (epsilon) chains on human IgE. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, mouse, rabbit serum proteins.
Isotype
IgE
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 – 1:2000
  • Immunomicroscopy 1:20 – 1:2000
Application Notes
This conjugate is suitable for immunomicroscopy and flow cytometry.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (epsilon) chains on human IgE.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with sterilized deionized water. Centrifuge to remove any particulates.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 650 (Ex = 652 nm; Em = 672 nm). After reconstituted, prepare fresh working dilution daily.

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Goat anti-Human IgE Secondary Antibody [DyLight 650] (Pre-absorbed)

  • IgE
  • immunoglobulin heavy constant epsilon

PMID: 19219047