Glypican 1 Antibody Summary
| Immunogen |
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Glypican 1/GPC1 protein.
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| Specificity |
This antibody is specific for human Glypican 1/GPC1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 – 88% identity with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
GPC1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This protein A purified antibody has been tested for use in ELISA and western blotting.Expect a band approximately ~61 kDa in size corresponding – glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight of transfected Fc-glypican compared with the expected MW of glypican is likely Due to the presence of the Fc-tag.
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Reactivity Notes
Hu, Mu, Rt + others
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Concentration |
LYOPH
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| Purity |
Immunogen affinity purified
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| Reconstitution Instructions |
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.
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Alternate Names for Glypican 1 Antibody
- FLJ38078
- Glypican 1
- glypican proteoglycan 1
- glypican
- glypican-1
- GPC1
Background
Glypican-1 (also known as Glypican 1/GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary
from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican 1/GPC1 has been shown to interact with SLIT2.
from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican 1/GPC1 has been shown to interact with SLIT2.