Glycerol Kinase Antibody [Biotin] Summary
| Description |
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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| Immunogen |
Glycerol Kinase [Cellulomonas species]
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
GK
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| Purity |
Delipidation and Defibrination
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This product has been assayed against 1.0 ug of Glycerol Kinase [Cellulomonas species] in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:500 – 1:2,500 of the reconstitution concentration is suggested for this product.
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| Readout System |
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Reactivity Notes
Reactive in Glycerol Kinase [Cellulomonas species]
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
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| Preservative |
0.01% Sodium Azide
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| Purity |
Delipidation and Defibrination
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Alternate Names for Glycerol Kinase Antibody [Biotin]
- ATP:glycerol 3-phosphotransferase
- EC 2.7.1.30
- GK
- GK1
- GKD
- glpK
- Glycerokinase
- Glycerol Kinase
Background
Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway.NB – Glycerol kinase obtained from Cellulomonas spp has an observed molecular weight of 128kDa.