Glycerol Kinase Antibody

Product: L-Chicoric Acid

Glycerol Kinase Antibody Summary

Immunogen
Synthetic peptides corresponding to GK (glycerol kinase) The peptide sequence was selected from the middle region of GK.Peptide sequence MAAGAAEGVGVWSLEPEDLSAVTMERFEPQINAEESEIRYSTWKKAVMKS.
Clonality
Polyclonal
Host
Rabbit
Gene
GK
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against GK and was validated on Western blot.
Reviewed Applications
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using
NBP1-57033 in the following application:

  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Glycerol Kinase Antibody

  • ATP:glycerol 3-phosphotransferase
  • EC 2.7.1.30
  • GK
  • GK1
  • GKD
  • glpK
  • Glycerokinase
  • Glycerol Kinase

Background

The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.

PMID: 24847734

Glycerol Kinase Antibody

Product: Sitagliptin

Glycerol Kinase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Glycerol Kinase [Cellulomonas species]
Clonality
Polyclonal
Host
Goat
Gene
GK
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 – 1:2000
  • ELISA 1:4000 – 1:20000
Application Notes
This product has been assayed against 1.0 ug of Glycerol Kinase [Cellulomonas species] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:4,000 – 1:18,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive in Glycerol Kinase [Cellulomonas species]

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Glycerol Kinase Antibody

  • ATP:glycerol 3-phosphotransferase
  • EC 2.7.1.30
  • GK
  • GK1
  • GKD
  • glpK
  • Glycerokinase
  • Glycerol Kinase

PMID: 21190016