Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [Alexa Fluor® 488] Summary
| Immunogen |
Recombinant human G6PD (35-506aa) purified from E. coli
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| Marker |
Cytosol Marker
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| Specificity |
This antibody is specific for human G6PD. This monoclonal antibody, clone 2F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human G6PD protein.
|
| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
G6PD
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
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| Preservative |
0.05% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [Alexa Fluor® 488]
- G6PD
- G6PD1EC 1.1.1.49
- glucose-6-phosphate dehydrogenase
Background
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). This full-length 545 aa form has been reported to be inactive, but may be processed to the smaller (515 aa) active form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.