Galectin-3 Antibody (B2C10) Summary
| Immunogen |
The antibody reacts with the Galectin-3 antigen.
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| Specificity |
The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3s hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
LGALS3
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The monoclonal antibody B2C10 can be used for Western blotting, flow cytometry and immunohistology on both frozen and paraffin embedded sections. Furthermore the monoclonal antibody B2C10 is useful for immuno assays and functional studies.For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS, containing 0.1% bovine serum albumin.
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| Preservative |
No Preservative
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| Concentration |
0.1 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for Galectin-3 Antibody (B2C10)
- AGE-R3
- Carbohydrate-binding protein 35
- CBP 35,35 kDa lectin
- CBP35
- GAL3
- Gal-3
- galactin-3
- Galactose-specific lectin 3
- Galactoside-binding protein
- GALBPCBP35
- Galectin3
- Galectin-3
- GALIG
- IgE-binding protein
- L29
- L31
- L-31
- Laminin-binding protein
- Lectin L-29
- lectin, galactoside-binding, soluble, 3
- LGALS2
- LGALS3
- Mac-2 antigen
- Mac-2
- MAC2GAL3
Background
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties.Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.