GRP78/HSPA5 Antibody (6H4-2G7) Summary
| Immunogen |
His-tagged human GRP78
|
| Localization |
Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome
|
| Marker |
ER Stress Marker
|
| Specificity |
Detects a protein with a mass of approx. 78kDa corresponding to GRP78
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
HSPA5
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
0.5 ug/ml of GRP78 Antibody was sufficient for detection of Grp78 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.
|
Reactivity Notes
Based on homology, It is predicted to detect Mouse, Rat, Bovine, Fungi, Chestnut Blight (Cryphonectria parasitica), Hamster, Monkey, Rabbit and Xenopus
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 50% Glycerol
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for GRP78/HSPA5 Antibody (6H4-2G7)
- 78 kDa glucose-regulated protein
- BIP
- Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
- FLJ26106
- GRP78
- GRP-78
- GRP78BIP
- Heat shock 70 kDa protein 5
- heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
- heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
- HSP70-5
- HSPA5
- Immunoglobulin heavy chain-binding protein
- MIF2
Background
0.5 ug/mL was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis.