GNAI3 Antibody

Product: FAS-IN-1 (Tosylate)

GNAI3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 3. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAI3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 – 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Mouse reactivity determined by WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.94 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GNAI3 Antibody

  • FLJ26559
  • G(i) alpha-3
  • guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6
  • guanine nucleotide-binding protein G(k) subunit alpha

Background

GAI3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase once its G protein coupled receptors (GPCR) such as a2 adrenergic receptors are activated. GAI3 can be inactivated by pertussis toxin. Guanine nucleotide binding proteins are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor regulated K(+) channels.

PMID: 2483273

GNAI3 Antibody

Product: PGD2-IN-3

GNAI3 Antibody Summary

Immunogen
GNAI3 (AAH25285.1, 1 a.a. – 354 a.a.) full-length human protein. MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY
Specificity
GNAI3 – guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3,
Clonality
Polyclonal
Host
Mouse
Gene
GNAI3
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. IHC usage reported in scientific literature (PMID: 25291362).
Publications
Read Publication using H00002773-B01P.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GNAI3 Antibody

  • FLJ26559
  • G(i) alpha-3
  • guanine nucleotide binding protein (G protein), alpha inhibiting activitypolypeptide 3,87U6
  • guanine nucleotide-binding protein G(k) subunit alpha

PMID: 21402875