GLI-2 Antibody Summary
| Immunogen |
Peptide with sequence DLKEDLDRDDCKQE corresponding to internal region according to NP_005261.2.
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| Specificity |
This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
GLI2
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Preliminary experiments gave an approx. 55 kDa band in human bone marrow and ovary lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa band according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. |
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| Publications |
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Reactivity Notes
Expected from sequence similarity:Mouse
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Notes
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.
Alternate Names for GLI-2 Antibody
- GLI family zinc finger 2
- GLI2
- GLI-2
- GLI-Kruppel family member GLI2
- glioma-associated oncogene family zinc finger 2
- HPE9tax helper protein 1
- oncogene GLI2
- tax helper protein 2
- Tax helper protein
- tax-responsive element-2 holding protein
- tax-responsive element-25-bp sequence binding protein
- THP
- THP1
- THP2
- zinc finger protein GLI2
Background
This protein belongs to the C2H2 type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes Greig cephalopolysyndactyly syndrome, Pallister Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.