GFAP Antibody Summary
| Immunogen |
Glial fibrillary acidic protein isolated from cow spinal cord.
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| Localization |
Astrocyte Marker
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| Marker |
Astrocyte Marker
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| Specificity |
In the central nervous system, this stains astrocytes and some groups of ependymal cells.
However, in the peripheral nervous system, it stains Schwann cells, satellite cells and enteric glial cells. |
| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
GFAP
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| Purity |
Protein A purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user.
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| Positive Control |
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Reactivity Notes
This antibody reacts with human, cat, chicken, cow, guinea pig, kangaroo, monkey, mouse, rat and sheep.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS
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| Preservative |
Sodium Azide
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| Purity |
Protein A purified
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Notes
This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.
Alternate Names for GFAP Antibody
- FLJ45472
- GFAP astrocytes
- GFAP
- glial fibrillary acidic protein
Background
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.