GCLC Antibody

Product: Tiratricol

GCLC Antibody Summary

Immunogen
A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)
Localization
Cytoplasmic
Specificity
Recognizes the heavy subunit of GCS at 73kDa
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCLC
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 2.5 – 5.0 ug/ml
  • Immunoprecipitation 10 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB600-544.

Reactivity Notes

Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for GCLC Antibody

  • EC 6.3.2.2
  • gamma-ECS
  • Gamma-glutamylcysteine synthetase
  • GCS heavy chain
  • GCS
  • GLCL
  • GLCLCGCL
  • glutamate–cysteine ligase catalytic subunit
  • glutamate-cysteine ligase, catalytic subunit

Background

Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit toheavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

PMID: 23318055