Filensin Antibody (FIL-7B10) Summary
| Immunogen |
Human and bovine lens filament enriched fraction (plasma membrane cytoskeleton complex).
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| Specificity |
The antibody recognizes an epitope located within either the rod or the N-terminal non-(a) helical domain of the filensin molecule. May be used in immunoblotting where it specifically detects both the full-length filensin (115 kDa), the processed fragment, and a variety of breakdown products in the mol. wt. range of 28-69 kDa. Does not react with cultured lens epithelial cells nor with normal human skin, psoriatic or tumoral skin (basal cell carcinoma).
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
BFSP1
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| Purity |
Unpurified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 115 kDa (full length filensin) and a band of approximately 53 kDa (processed filensin fragment). It also reacts with a variety of breakdown products of filensin in the range of 28-69 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
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Reactivity Notes
Cross-reacts with Human, Cow and Sheep.Does not cross-react with Mouse, Rat or Chicken.Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
Ascites
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| Preservative |
15mM Sodium Azide
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| Purity |
Unpurified
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Alternate Names for Filensin Antibody (FIL-7B10)
- Beaded filament structural protein 1
- beaded filament structural protein 1, filensin
- CP115Lens intermediate filament-like heavy
- filensin
- Lens fiber cell beaded-filament structural protein CP 115
- LIFL-H115 KD
Background
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, CP49 (also known as phakinin) and filensin (or CP115), are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF).