Filamin A Antibody (PM6/317)

Product: CDK9-IN-7

Filamin A Antibody (PM6/317) Summary

Immunogen
Purified human platelet plasma membrane
Specificity
Recognizes both purified Filamin A, and a 250kD protein in cell lysates. Clone PM6/317 has also been reported to work on Western blotting.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLNA
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:5
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Membrane permeabilisation is required for Flow Cytometry.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for Filamin A Antibody (PM6/317)

  • ABP-280DKFZp434P031
  • ABPX
  • actin binding protein 280
  • Actin-binding protein 280
  • alpha-filamin
  • CVD1
  • EC 2.1.1.43
  • EC 6.3.4.4
  • Endothelial actin-binding protein
  • filamin A, alpha (actin binding protein 280)
  • filamin A, alpha
  • filamin-1
  • filamin-A
  • FLN
  • FLN1OPD
  • FLN-A
  • FMD
  • MNS
  • NHBP
  • Non-muscle filamin
  • OPD1
  • OPD2
  • XLVD
  • XMVD

Background

Filamin A is an actin-binding protein that crosslinks and promotes orthogonal branching of actin filaments and also functions to anchor transmembrane proteins and intracellular signaling proteins to the actin cytoskeleton. As a scaffolding protein, Filamin A is able act as a link between cell membrane receptors and intracellular signaling proteins. Defects in Filamin A are associated with multiple human diseases characterized by abnormalities in bone and neurological development.

PMID: 22505612