Product: 4,7-Dicaffeoylquinic acid
Fibronectin/Anastellin Antibody (A32) – BSA Free Summary
| Immunogen |
Bovine corneal endothelial cells
|
| Epitope |
Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of Clone A32
|
| Marker |
Mesenchymal Cells Marker
|
| Specificity |
Clone A32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). Clone A32 cross-reacts with human fibronectin. Other species have not been tested.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FN1
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Clone A32 can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody Clone A32. In Western blotting a dilution guideline of 1/100 has proved successful.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
10mM Phosphate (pH 7.4) and 0.5M NaCl
|
| Preservative |
15mM Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for Fibronectin/Anastellin Antibody (A32) – BSA Free
- CIGDKFZp686O13149
- Cold-insoluble globulin
- DKFZp686F10164
- DKFZp686H0342
- DKFZp686I1370
- fibronectin 1
- fibronectin
- Fibronectin/Anastellin
- FNFINC
- FNZ
- GFND2ED-B
- LETSGFND
- migration-stimulating factor
- MSF
Background
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities