Fibrillarin Antibody (28F2) Summary
| Immunogen |
Yeast nuclear preparation (S. cerevisiae).
|
| Marker |
Nucleolar Marker
|
| Isotype |
IgG3
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FBL
|
| Purity |
Tissue culture supernatant
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This Fibrillarin antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Yeast.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
No buffer
|
| Preservative |
No Preservative
|
| Purity |
Tissue culture supernatant
|
Alternate Names for Fibrillarin Antibody (28F2)
- EC 2.1.1,34-kD nucleolar scleroderma antigen
- EC 2.1.1.-
- EC 2.1.1.37
- FIB
- FIB1,34 kDa nucleolar scleroderma antigen
- fibrillarin
- FLRNrRNA 2-O-methyltransferase fibrillarin
- RNA, U3 small nucleolar interacting protein 1
- RNU3IP1
Background
Fibrillarin (FBL; human homologue of yeast Nop1p) is the most abundant proteins in fibrillar regions of the eukaryotic cell nucleolus, the site for early phase of pre-rRNA processing, and is considered an excellent marker for nucleolus. FBL interacts with several proteins such as NOLC1, DDX5, C1QBP, PRMT5 and UTP20; and with U3, U8, U13, X and Y small nuclear RNAs also. FBL forms a component of several ribosomal/nucleolar protein complexes and of snoRNP (box C/D small nucleolar ribonucleoprotein) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. In pre-ribosomal RNA, FBL utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2-hydroxyl methylation of ribose moieties, the site specificity being offered by the guide RNA that base pairs with the substrate, and the methylation occurs at a unique distance from the sequence involved in base pairing with the guide RNA. In humans, FBL also acts as a nucleolar autoantigen for non-hereditary immune disease scleroderma, a disease in which the patients have strong circulating FBL autoantibodies.