FTO Antibody (5-2H10) Summary
| Immunogen |
Synthetic peptide from the N-terminal region of rat FTO
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| Specificity |
Specific for the ~58 kDa FTO protein in Western blots of rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
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| Predicted Species |
Canine (100%), Equine (100%), Sheep (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
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| Isotype |
IgG
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FTO
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| Purity |
Protein G purified
|
| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
|
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
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| Preservative |
No Preservative
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| Purity |
Protein G purified
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Alternate Names for FTO Antibody (5-2H10)
- Alpha-ketoglutarate-dependent dioxygenase FTO
- EC 1.14.11.-
- fat mass and obesity associated
- Fat mass and obesity-associated protein
- FTO
- KIAA1752protein fto
- MGC5149
Background
The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009)