FITC Antibody (931845) [Unconjugated] Summary
| Immunogen |
Fluorescein Isothiocyanate (FITC)
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| Specificity |
Detects Fluorescein Isothiocyanate (FITC) and Carboxyfluorescein (CFS) in direct ELISAs. In direct ELISAs, no cross-reactivity with Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 647, Alexa Fluor® 700, Alexa Fluor® 750, NorthernLights™ NL493, NorthernLights™ 557, and NorthernLights™ 637 is observed.
|
| Source |
N/A
|
| Isotype |
IgG
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
|
| Preservative |
No Preservative
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| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS.
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Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for FITC Antibody (931845) [Unconjugated]
- FITC
- Fluorescein Isothiocyanate
Background
Fluorescein isothiocyante (FITC) is a derivative of fluorescein modified with an isothiocyanate reactive group (-N=C=S). FITC is often used to label antibodies for immunofluorescent cytochemistry and histochemistry. FITC can be also conjugated to proteins and RNA and DNA probes because such conjugation does not impair biological activity of these molecules. In some experimental setups FITC fluorescence may need to be enhanced, which is done by employing anti-FITC antibodies conjugated either to FITC or other fluorophores with similar excitation and emission spectra.