EphB1 [p Tyr317] Antibody

Product: Flupirtine (Maleate)

EphB1 [p Tyr317] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB. Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.
Modification
p Tyr317
Specificity
Specific for the ~46k EphrinB protein phosphorylated at Tyr317. The immunolabeling of the EphrinB band is blocked by treatment with lambda-phosphatase.
Predicted Species
Human (100%), Xenopus (100%), Bovine (100%), Chicken (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
EPHB1
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa. Immunofluorescence (PMID: 21530605)
Publications
Read Publication using
NB300-275 in the following applications:

  • ICC/IF
    1 publication
  • WB
    1 publication

Reactivity Notes

Tested in Rat. Mouse reactivity reported in scientific literature (PMID: 21539827).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for EphB1 [p Tyr317] Antibody

  • Cek6
  • EC 2.7.10
  • EC 2.7.10.1
  • EK6
  • Elk
  • EPH receptor B1
  • eph tyrosine kinase 2
  • EphB1
  • EPH-like kinase 6
  • ephrin type-B receptor 1
  • EPHT2FLJ37986
  • Hek6
  • Net
  • NETHek6
  • soluble EPHB1 variant 1
  • Tyrosine-protein kinase receptor EPH-2

Background

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

PMID: 25248068