EpCAM/TROP1 Antibody (SPM491)

Product: HOKU-82

EpCAM/TROP1 Antibody (SPM491) Summary

Immunogen
Recombinant full-length human EpCAM protein
Localization
Cell Surface & Cytoplasmic
Marker
Epithelial Marker
Specificity
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TACSTD1, EPCAM
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 – 1.0 ug/ml
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml
  • Immunofluorescence 1 – 2 ug/ml
Application Notes
Hu-chromosome location: 2p21
Molecular weight of antigen: 40-43kDa
Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for EpCAM/TROP1 Antibody (SPM491)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

PMID: 25118166