EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) – Azide and BSA Free

Product: Cariprazine

EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) – Azide and BSA Free Summary

Immunogen
Recombinant human Ep-CAM protein (full-length and fragments)
Localization
Cell surface
Marker
Epithelial Marker
Specificity
It is a cocktail of four highly specific monoclonal antibodies (EGP40/826, EGP40/837, EGP40/1110, EGP40/1120) that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or Ep-CAM. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TACSTD1, EPCAM
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot : 0.5 – 1.0 ug/ml
  • Flow Cytometry : 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin : 0.5 – 1.0 ug/ml
  • CyTOF-ready
  • Immunofluorescence : 1 – 2 ug/ml
Application Notes
Hu-chromosome location: 2q21 Molecular weight of antigen: 40-43kDa Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) – Azide and BSA Free

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

PMID: 22700794