ER alpha/NR3A1 Antibody (2B7E3)

Product: Q-VD-OPh

ER alpha/NR3A1 Antibody (2B7E3) Summary

Immunogen
Ni-NTA purified recombinant human ER-alpha (410-592aa) expressed in E. Coli strain BL21 (DE3)
Specificity
ER-alpha (2B7E3)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 – 1:500
  • ELISA 1:10000
  • Immunohistochemistry 1:200 – 1:1000
  • Immunohistochemistry-Frozen 1:100 – 1:500
  • Immunohistochemistry-Paraffin 1:100 – 1:500
Positive Control
ER alpha/NR3A1 Lysate (NBL1-10344)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Purity
Protein A or G purified

Alternate Names for ER alpha/NR3A1 Antibody (2B7E3)

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.

PMID: 23537664