EMI1 Antibody Summary
| Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human Fbp5A protein.
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| Marker |
Mitosis Marker
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| Specificity |
This antibody is directed against human Fbp5A protein. A BLAST analysis was used to suggest cross reactivity with Fbp5A protein.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
FBXO5
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Expect a band approximately 51 kDa in size corresponding – Fbp5A protein by western blotting in the appropriate cell lysate or extract.
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Reactivity Notes
Reactive in
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for EMI1 Antibody
- EMI1Early mitotic inhibitor 1
- F-box only protein 5
- F-box protein 5
- FBX5F-box protein Fbx5
- Fbxo31
Background
FBXO5 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class and is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.