ECSIT Antibody

Product: Acetylcholine (chloride)

ECSIT Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human ECSIT protein.
Specificity
This antibody is directed against human ECSIT protein.   A BLAST analysis was used to suggest cross reactivity with ECSIT protein from human and mouse.
Clonality
Polyclonal
Host
Rabbit
Gene
ECSIT
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:20000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Expect a band approximately 45 kDa in size corresponding – ECSIT protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in human, mouse

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ECSIT Antibody

  • ECSIT homolog (Drosophila)
  • evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
  • likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
  • Protein SITPEC
  • signaling intermediate in Toll pathway evolutionarily conserved ortholog
  • SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Background

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

PMID: 9121605

ECSIT Antibody

Product: Lurasidone Metabolite 14283 D10

ECSIT Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human ECSIT. The immunogen is located within amino acids 290 – 340 of ECSIT.
Clonality
Polyclonal
Host
Rabbit
Gene
ECSIT
Purity
Ion exchange chromatography
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ECSIT antibody can be used for detection of ECSIT by Western blot at 0.5 to 1 mg/ml.
Control Peptide
ECSIT Peptide (NBP1-76594PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for ECSIT Antibody

  • ECSIT homolog (Drosophila)
  • evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
  • likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
  • Protein SITPEC
  • signaling intermediate in Toll pathway evolutionarily conserved ortholog
  • SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Background

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

PMID: 21571946