Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP]

Product: Cromolyn (sodium)

Donkey anti-Rabbit IgG (H L) Secondary Antibody [HRP] Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

PMID: 7515954