Donkey anti-Mouse IgG Secondary Antibody [Texas Red]

Product: PQ401

Donkey anti-Mouse IgG Secondary Antibody [Texas Red] Summary

Immunogen
Mouse IgG whole molecule
Specificity
Mouse IgG (H and L)
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 – 1:10000
  • Flow Cytometry 1:1:10 – 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 – 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 – 1:5000
Application Notes
Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Donkey anti-Mouse IgG Secondary Antibody [Texas Red]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

PMID: 17374608