Donkey anti-Mouse IgG (H+L) Secondary Antibody [DyLight 550]

Product: Kaempferol

Donkey anti-Mouse IgG (H L) Secondary Antibody [DyLight 550] Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. No reactivity is observed to non-immunoglobulin mouse serum immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20 – 1:2000
  • Immunomicroscopy 1:20 – 1:2000
Application Notes
Antibody is > 95% based on SDS-PAGE.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Notes

Affinity purified antibody is > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. DyLight 550 (Ex = 550 nm; Em = 576 nm)

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

PMID: 15974572