D-Dimer Antibody (DD2) Summary
Immunogen |
This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.
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Specificity |
this is more specific to high molecular weight fibrin degradation products than to D-dimer. No cross-reaction with D-monomer.
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Isotype |
IgG2b
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Clonality |
Monoclonal
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Host |
Mouse
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Purity |
Protein A purified
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Applications/Dilutions
Dilutions |
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Application Notes |
Interacts with beta-chain of D-dimer in Western blotting under reducing conditions. Recommended pairs to be used in a one-step sandwich immunoassay for D-dimer detection in human plasma (capture-detection): NB110-8376 – NB100-73038 and NB110-8376 – NB100-73039, NB110-8376 – NB120-10055. To be analyzed in a two-step sandwich immunoassay, plasma must be diluted with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.
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Reactivity Notes
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze.
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Buffer |
PBS (pH 7.4)
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Preservative |
0.09% Sodium Azide
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Purity |
Protein A purified
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Alternate Names for D-Dimer Antibody (DD2)
- D Dimer
- Ddimer
- D-dimer
- FDP