D-Dimer Antibody (DD2)

Product: Regadenoson

D-Dimer Antibody (DD2) Summary

Immunogen
This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot.
Specificity
this is more specific to high molecular weight fibrin degradation products than to D-dimer. No cross-reaction with D-monomer.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoassay
  • Sandwich ELISA 1:100-1:2000
Application Notes
Interacts with beta-chain of D-dimer in Western blotting under reducing conditions. Recommended pairs to be used in a one-step sandwich immunoassay for D-dimer detection in human plasma (capture-detection): NB110-8376 – NB100-73038 and NB110-8376 – NB100-73039, NB110-8376 – NB120-10055. To be analyzed in a two-step sandwich immunoassay, plasma must be diluted with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for D-Dimer Antibody (DD2)

  • D Dimer
  • Ddimer
  • D-dimer
  • FDP

Background

A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process.

PMID: 19584159