DNA Antibody (AE-2) – IHC-Prediluted

Product: CVT-314

DNA Antibody (AE-2) – IHC-Prediluted Summary

Immunogen
Nuclei of Burkitts cells
Localization
Nuclear
Marker
Nuclear Marker
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3,5-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Immunocytochemistry Acetone-fixed cells 0.5 – 1.0 ug/ml for 30 minutes at RT. Immunohistochemistry-Paraffin 1 – 2 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for DNA Antibody (AE-2) – IHC-Prediluted

  • Double stranded deoxyribonucleic acid
  • Double stranded DNA
  • dsDNA

PMID: 25257174